. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MELANOPHORE RESPONSE TO COLCHICINE 229 hydrochloride. The action of colchicine was studied in relation to its effect on the response of the melanophores to epinephrine. Epinephrine was used because it produces rapid aggregation of the melanin in Fiindulus melanophores (Spaeth and Barbour, 1917) and has been shown to act directly on the fish melanophore (Fujii, 1961). MATERIALS AND METHODS Experiments were performed with isolated scales from mature specimens of the killifish, Fundulus heteroclitus. The fishes were obtained fr
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. MELANOPHORE RESPONSE TO COLCHICINE 229 hydrochloride. The action of colchicine was studied in relation to its effect on the response of the melanophores to epinephrine. Epinephrine was used because it produces rapid aggregation of the melanin in Fiindulus melanophores (Spaeth and Barbour, 1917) and has been shown to act directly on the fish melanophore (Fujii, 1961). MATERIALS AND METHODS Experiments were performed with isolated scales from mature specimens of the killifish, Fundulus heteroclitus. The fishes were obtained from the Marine Biological Laboratory, Woods Hole, Massachusetts. They were kept in an "Instant Ocean" Culture System in artificial sea water which was maintained between 10 and 13° C. For each experiment scales were removed with fine forceps from the anterior dorsal and dorsolateral regions of the fish. The scales were mounted on a glass coverslip which was sealed to a Dick perfusion chamber (Dick, 1955). The scales were mounted and the chamber was sealed with Vaspar (4 parts beeswax to 1 part yellow vaseline). Ringer's solution was perfused through the 10 15 TIME (MINUTES) 20 25 FIGURE 1. The effects of colchicine pretreatment for 40 minutes on the response of melano- phores to mM epinphrine. Points are means ± standard errors. (O) Ringer, 18 scales; (•) mM colchicine, 8 scales; (A) 5 mM colchicine, 10 Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
