. The elements of pathological histology with special reference to practical methods . yanide, and then in glycerin containing a \ per cent, of aceticacid, when pigment containing iron becomes blue. If nuclear staining is alsodesired, the sections, after washing in water, are further treated with alumcochineal ; or an alternative method is to immerse them at the commencementin a solution of lithium carmine to which some drops of the potassium ferrocy-anide solution have been added, whence they are transferred to hydrochloricacid glycerin (p. 20), or washed in hydrochloric acid alcohol (p. 18).
. The elements of pathological histology with special reference to practical methods . yanide, and then in glycerin containing a \ per cent, of aceticacid, when pigment containing iron becomes blue. If nuclear staining is alsodesired, the sections, after washing in water, are further treated with alumcochineal ; or an alternative method is to immerse them at the commencementin a solution of lithium carmine to which some drops of the potassium ferrocy-anide solution have been added, whence they are transferred to hydrochloricacid glycerin (p. 20), or washed in hydrochloric acid alcohol (p. 18). 2. The sections are left in freshly-prepared ammonium sulphide until theyhave turned blackish-green, which requires from five to twenty minutes ; theyare then washed in water and mounted, either in glycerin containing someammonium sulphide, or in Canada balsam. The pigment assumes a blackish-green or black colour. CALCIFICATION 61 It need hardly be said that in carrying out the test-staining for iron allcontamination with that substance, such as might come from preparation needles. FlO. SUB-PAPILLABY STRATUM OK THE MuCOUS MEMBRANE OF THE TONGUE. X 285. (Stained in alum cochineal.) a, Vein; 6, Granules of silver in the wall of the veinand its immediate neighbourhood ; c, Fibrinous coagulum ; d, Red corpuscles. or from the knife used for cutting, must be avoided ; and it should be furtherborne in mind that ammonium sulphide blackens other metals also, such as lead,mercury, and silver. 6. Calcification.—This as a rule takes place only in necrosedmasses, or in tissues which are at least in a lowered state of nutri-tion, but it also occurs in unaltered tissues in cases of extensiveabsorption of bone {metastatic calcification). It affects the cellsas well as the interstitial substance. The lime deposited—chieflycalcium phosphate and carbonate—usually appears at first in theform of minute granules, black by transmitted (Fig. 26, cl), but shiningwhite by reflected
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Keywords: ., bookcentury1800, bookdecade1890, booksubjectpatholo, bookyear1895
