. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PH Figure 4. The effects of L-lactate on HcCK binding at 5°C of inter- mediate instars of Gnathophausia ingens from California. Regression equations for HcO; affinity: mmol T1 L-lactate, Log Ps0 = - pH, r = ; L-lactate, Log P50 = - pH, r = ; L-lactate, Log P50 = - pH. r = Regres- sion equations for cooperativity (lines plotted for and mmol/1 L-lactate): mmol 1~' L-lactate. nso = + pH, r = : L-lactate, n50 = -2.
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. PH Figure 4. The effects of L-lactate on HcCK binding at 5°C of inter- mediate instars of Gnathophausia ingens from California. Regression equations for HcO; affinity: mmol T1 L-lactate, Log Ps0 = - pH, r = ; L-lactate, Log P50 = - pH, r = ; L-lactate, Log P50 = - pH. r = Regres- sion equations for cooperativity (lines plotted for and mmol/1 L-lactate): mmol 1~' L-lactate. nso = + pH, r = : L-lactate, n50 = + pH, r = ; L- lactate, nM = + pH, r = Ol o o m C 08 06 04 02 00 -02 -04 3 2 1. 70 76 pH 78 Figure 5. HcO: affinity and cooperativity at 5°C in fresh and frozen hemolymph from intermediate instar and brooding female Giuilho- phuiixia 'ii.'i from California, and from intermediate instar Hawai- ian G <,rm. Regressions for each affinity line: California fresh (fresh). Log P50 = - pH, r = ; California frozen (frozen). Log P50 = - pH, r = ; California brooding female frozen (brood). Log P50 = - pH, r = ; Hawaii frozen (Hawaii), Log P50 = - pH, r = .99. Regressions for cooperativity (lines for California fresh and California frozen): California fresh (fresh), nso + pH. r = ; California frozen (frozen), n50 = + pH, r = ; California brooding female frozen (brooding). n50 pH, r = ; Hawaii frozen (Hawaii), n50 = + pH, r = .99 si a ingens that had been stored at -SOT for 18-24 months contained hemocyanin with significantly in- creased O: affinity (5°C P < , ANCOVA, Fig. 5) and significantly decreased cooperativity (P < , AN- COVA). Therefore, we used hemolymph that had never been frozen for all of the other studies on the HcO: bind- ing properties of intermediate instar individuals from California. However, because of th
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
