. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 102 S. IKEGAMI ET AL. c nj n 0 25 0 20 0 10 0 05. 0013 0025 0050 0100 Number of individuals per well Figure 9. Measurement of antigen content in extracts of Asterma peclinifera oocytes (O), eggs(»), 1-day-old early gastrulae (A), 3-day-old late gastrulae (A), and 4-day-old early hipinnariae (D) using an ELISA system with the Kl antibody. The method of measurement of antigen content is described in Materials and Methods. inhibition test, using rabbit pectinij'era hexokinase antisera. The antisera inhibited the activ
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 102 S. IKEGAMI ET AL. c nj n 0 25 0 20 0 10 0 05. 0013 0025 0050 0100 Number of individuals per well Figure 9. Measurement of antigen content in extracts of Asterma peclinifera oocytes (O), eggs(»), 1-day-old early gastrulae (A), 3-day-old late gastrulae (A), and 4-day-old early hipinnariae (D) using an ELISA system with the Kl antibody. The method of measurement of antigen content is described in Materials and Methods. inhibition test, using rabbit pectinij'era hexokinase antisera. The antisera inhibited the activity from hexo- kinase of A. bat/ieri, Asterina coronata japonica, and A. pseudoexigua pacifica by 50-54% and that of A. minor by 30%. In light of these results, Mochizuki and Hori suggested that A. pectinij'era and A. minor, but not the other Astrina species, belong to convergent lineages. Mat- suoka (1981) arrived at a similar conclusion as a result of electrophoretic analysis of hexokinase and six other en- zymes from five Asterina species. In our study, a peptide capable of recognizing the Kl monoclonal antibody, which is reactive to A. pectinifera p58/56, was found only in embryos and larvae of A. pseudoexigua pacifica among the Asterina species examined. Since little information is available regarding the function and structure of the pep- tides containing the K1 epitope, the reason for the phy- logenetic variation of the peptides in these two species remains unclear. So far, A. pseudoexigua pacifica has been found only in Sabiura, ofFKushimoto in Wakayama Prefecture (Ko- matsu el a!., 1990). It is tiny (its body is only 5 mm long) and ovoviviparous. Development of this species, from fertilization to the completion of metamorphosis, occurs within the gonad. The juveniles, measuring approximately mm in total length, crawl out of the gonad and pass through the gonoduct. According to the observation of Komatsu and Kano, made in July and August of 1972, the maximum number
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