. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. IONIC CURRENTS IN MOUSE EMBRYOS 113 '•• „. lOna/rni2. Figure 2. (a) Parasaggital view of embryo in the recording chamber with the computer vectors superimposed over the video image. The mouse embryo is held from the right with a fire-polished holding micropipet. The IBM-XT computer displays the current vector at each position. The magnitude of the current is represented by the length of the vector, while the direction of the vector shows the current direction. Note that maximum outward current is detected ove
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. IONIC CURRENTS IN MOUSE EMBRYOS 113 '•• „. lOna/rni2. Figure 2. (a) Parasaggital view of embryo in the recording chamber with the computer vectors superimposed over the video image. The mouse embryo is held from the right with a fire-polished holding micropipet. The IBM-XT computer displays the current vector at each position. The magnitude of the current is represented by the length of the vector, while the direction of the vector shows the current direction. Note that maximum outward current is detected over the developing foregut (fg). As the probe is moved both anterior and posterior to the foregut the outward current decreases. Anterior to the foregut. an arrow marks the juncture between the embryonic and extraembryonic region. At this juncture note how the outward current abruptly reverses direction and the current vectors now point inward. Posterior to the hindgut (hg) note how the current vectors gradually begin to turn inward, representing an inward ionic current. Bar = mm. (b) Three current vectors recorded from a embryo in frontal orienta- tion. The foregut is in the foreground, while the hindgut is hidden. The current vectors indicate that the maximal current is outward along the midline, and falls ofTsharply as the probe is moved laterally towards the yolk sac (ys). Bar = mm. ATPase inhibitor (Borland and Tasca, 1974), would in- terfere with the current pattern. In our initial experi- ments, we added 100 iiM ouabain to the media, after we had done a control recording of the current pattern. However, we observed no change in the current pattern even after a few hours of exposure to ouabain. Similarly, culture of embryos in 100 pM ouabain had no develop- mental effect on the embryos. We also attempted to use amiloride to block Na+ channels and the Na+/H+ trans- porter (Simchowitz and Cragoe, 1986). After an 18-min stable recording period with the pr
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
