. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FIG. 1. Gross appearance of the egg-agglutination reaction. All photographs natural size. (A) Five drops Arbacla eggs in 3 cc. of 5 per cent sperm extract. Note the drops of agglutinated eggs suspended from the surface of the solution. (B) Ten drops of Arbacia eggs in 3 cc. of 5 per cent sperm extract. The tube was shaken once. The eggs have formed a large dense mass which floats at the surface. (C) Ten drops of Arbacla eggs in 3 cc. heated sea water. The tube was shaken once after adding the eggs. Observe that the ova are h
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. FIG. 1. Gross appearance of the egg-agglutination reaction. All photographs natural size. (A) Five drops Arbacla eggs in 3 cc. of 5 per cent sperm extract. Note the drops of agglutinated eggs suspended from the surface of the solution. (B) Ten drops of Arbacia eggs in 3 cc. of 5 per cent sperm extract. The tube was shaken once. The eggs have formed a large dense mass which floats at the surface. (C) Ten drops of Arbacla eggs in 3 cc. heated sea water. The tube was shaken once after adding the eggs. Observe that the ova are homogeneously suspended and have begun to settle as shown by the clear area immediately below the surface. The details of the egg agglutination reaction may be observed micro- scopically by making an egg suspension in Chinese ink which reveals the colorless jelly layer, or chorion, surrounding each ovum. By means of a capillary pipette, sperm extract is introduced into a drop of this mixture mounted on a slide beneath a raised coverslip and observed under high power. As the extract comes in contact with an egg, a faint black line of adhering ink particles immediately appears at the periphery of the jelly. This line rapidly increases in thickness until the chorion is encircled by a dense membranous structure which will be termed the " agglutination ;. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
