Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microörganisms . Fig. 33-—Microscopic appear-ances of colonies. Fig. 34.—Klatsch preparations. they have received, or the greater or less amount of spacethat they have had to develop in. The microscopic picture varies greatly; now it is like thegnarled roots of a tree, and now like bits of frosted glass;some bacteria have quite characteristic colonies (Fig. 32). Impression or Klatsch Preparations.—In ordermore thoroughly to study a certain colony and to make apermanent specimen of the s
Essentials of bacteriology; being a concise and systematic introduction to the study of bacteria and allied microörganisms . Fig. 33-—Microscopic appear-ances of colonies. Fig. 34.—Klatsch preparations. they have received, or the greater or less amount of spacethat they have had to develop in. The microscopic picture varies greatly; now it is like thegnarled roots of a tree, and now like bits of frosted glass;some bacteria have quite characteristic colonies (Fig. 32). Impression or Klatsch Preparations.—In ordermore thoroughly to study a certain colony and to make apermanent specimen of the same, we press a clean cover-glassupon the particular colony, and it adheres to the glass. Itcan then be stained or examined. The Germans give thename of Klatsch to such preparations. Fishing.—To obtain and examine the individual members THE GROWTH AND APPEARANCES OF COLONIES 89 of a particular colony the process of fishing, as it is called, isresorted to. The colony having been placed under the field of the micro-. Fig. 35.—Types of growth in stab-cultures: A, Non-liquefying: i,Filiform (Bacillus coli); 2, beaded (Streptococcus pyogenes); 3, echinate(Bacterium acidi lactici); 4, viUous (Bacterium murisepticum); 5, arbor-escent (Bacillus mycoides). B, Liquefying: 6, Crateriform (Bacillusvulgare, twenty-four hours); 7, napiform (Bacillus subtihs, forty-eighthours); 8, infundibuhform (Bacillus prodigiosus); 9, saccate (Micro-sporon Finkleri); 10, stratiform (Psorospermum fluorescens) (Frost). scope, a long platinum needle, the point slightly bent, ispassed between the lens and the plate so as to be visiblethrough the microscope, then turned downward until thecolony is seen to be disturbed, and the needle is dipped into 90 ESSENTIALS OF BACTERIOLOGY the colony. This procedure must be carefully done, lest adifferent colony be disturbed than the one looked at, and anunknown or unwanted germ obtained. After the needle has entered the particular colony, it iswithdrawn, and t
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