Electron microscopy; proceedings of the Stockholm Conference, September, 1956 . electronmicrosco00euro Year: 1957 Fig. 1. Section of intact cells of Bacillus M. Magnification >: 28,000. Fig. 2. Section of protoplasts of Bacillus M. Magnification X 38,000. Fig. 3. Section of 'ghosts' of Bacillus M. Magnification 25,000. Fig. 4. Electron micrograph of drop preparation of 'ghost' of Bacillus M. acid found in genuine B. nwi^atcrium strains. They seem instead to have more in common with the non-lipid granules that have recently been isolated by Georgi, Militzer and Decker (2) from the ther- mop
Electron microscopy; proceedings of the Stockholm Conference, September, 1956 . electronmicrosco00euro Year: 1957 Fig. 1. Section of intact cells of Bacillus M. Magnification >: 28,000. Fig. 2. Section of protoplasts of Bacillus M. Magnification X 38,000. Fig. 3. Section of 'ghosts' of Bacillus M. Magnification 25,000. Fig. 4. Electron micrograph of drop preparation of 'ghost' of Bacillus M. acid found in genuine B. nwi^atcrium strains. They seem instead to have more in common with the non-lipid granules that have recently been isolated by Georgi, Militzer and Decker (2) from the ther- mophilic bacterium Bacillus stcarothcrniophilus. These workers report that the granules contain desoxyribonucleic acid among other things and, from the enzymological point of view, cytochrome c and phosphatases. It should, however, be borne in mind that the granules found in the bacterial 'ghosts' may be artefacts. The osmotic lysis must be regarded as a rather violent disintegration process, readily causing rearrangements between the protoplasmic constitu- ents of the bacterial cell. Such an interpretation is strengthened by the fact that no granules of the size and the electron density found in the 'ghosts' of Bacillus M, are seen in sections of intact cells or protoplasts. References Brenner, S. and Stent, G., Bioc/iim. Bioplivs. Acta 17, 473 (1955). Georgi, C. E., Militzer, W. E., and Decker, T. S., J. Bactoriol. 70, 716 (1955). Landman, O. E. and Spiegelman, S., Pioc. Natl. Acad. Sci. Wash. 41, 698 (1955). McQuillen, K., Biochim. Biophys. Acta 17, 382 (1955). ^ /. Gen. Microbiol. 13, iv (1955). ^ Biochim. Biophys. Acta 18, 458 (1955). Mutsaars, W., Ann. Inst. Pasteur 89, 166 (1955). , M. R. J., Nature 170, 746 (I952J. — /. Gen. Microbiol. 13, iv (1955). Salton, M. R. J. and McQuillen, K., Biochim. Biophvs. Acta 17, 465 (1955). ToMCSiK, J., Symp. Soc. Gen. Microbiol. 6, 53 (1955). ToMcsiK, J. and Guex-Holzer, S., Schwei:. Z. allgcm. Pathol, u. Bakteriol. 15, 517 (1952
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