. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 500 C. S. SIKES AND A. P. WHEELER TABLE II i>fC-14 labeled matrix into whole embryos and isolated spicules , < 'a/!, Arbacia punctulata Control 2°C (means ± SD) Experimental 22°C (means ± SD) jtg matrix/ ^mole CaCO3* C-14 matrix in whole embryos, DPM/104 embryos C-14 matrix in isolated spicules, DPM/104 embryos 3190 ± 107 ± 8232 ± 747 113 ± * (Expt'l - Control DPM/104 embs) (^g matrix/DPM) (104 ^mole CaCO3). There were jug matrix/ml of medium giving a radioactivity of 59,600 ±111
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 500 C. S. SIKES AND A. P. WHEELER TABLE II i>fC-14 labeled matrix into whole embryos and isolated spicules , < 'a/!, Arbacia punctulata Control 2°C (means ± SD) Experimental 22°C (means ± SD) jtg matrix/ ^mole CaCO3* C-14 matrix in whole embryos, DPM/104 embryos C-14 matrix in isolated spicules, DPM/104 embryos 3190 ± 107 ± 8232 ± 747 113 ± * (Expt'l - Control DPM/104 embs) (^g matrix/DPM) (104 ^mole CaCO3). There were jug matrix/ml of medium giving a radioactivity of 59,600 ±1110 DPM/min (n = 12). The culture contained 5020 ± 599 embryos/ml (n = 10) in 12 ml of total volume. of embryos/ml was raised to 11,400 ± 1230 (n = 18) to allow a rapid measurement of O2 consumption. This did not seem to affect the embryos adversely, in that the overall amount of O2 consumption measured was ± nmoles O2/1000 em- bryos/h (n = 9). This falls into the range of values of respiration by urchin embryos reported by other workers (Yanagisawa, 1975). The value for O2 consumption was computed based on the slope of the curves over the first 15 minutes of an assay. As seen in Figure 4, after this time, the rate of O2 consumption began to decrease, possibly due to damage to the embryos caused by the stirring needed to optimize the response of the oxygen electrode. 125 E 100 c ra ±E 75 I O CO 50 25 - 6 Minutes FIGURE 2. The incorporation of C-14 labeled matrix during CaCO3 formation in vitro in the pH-stat crystallization assay. C-14 labeled matrix was added at n%/m\ ( X 103 DPM/ml) after 25 of NaOH titrant had been delivered by Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laborat
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
