. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 276 SIIIKi ) IIORIUCH] AND i HARLES E. LANE 111 activity oj the < rystalh ^trombus i;/i;«.v wiili unions substrates Substrate Viscosity fig. glucose/mg. of enzyme In. CMC To low 30 cps. CMC 70 mol. »7o cps. CMC 70 hiiili 2100 cps. CMC" «)() 280 cps. CMC 120 h 1 .-55 cps. Suollen filter paper ( Yllulo-r powder . • Incubation at so and pi I lor CMC, pH for other substrates. The optimum temperature and the temperature of inactivation of the enzyme so


. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 276 SIIIKi ) IIORIUCH] AND i HARLES E. LANE 111 activity oj the < rystalh ^trombus i;/i;«.v wiili unions substrates Substrate Viscosity fig. glucose/mg. of enzyme In. CMC To low 30 cps. CMC 70 mol. »7o cps. CMC 70 hiiili 2100 cps. CMC" «)() 280 cps. CMC 120 h 1 .-55 cps. Suollen filter paper ( Yllulo-r powder . • Incubation at so and pi I lor CMC, pH for other substrates. The optimum temperature and the temperature of inactivation of the enzyme solution were determined. The relationship between the incubation temperature and cellulase activity showing an optimum about 40° C. is presented in Figure 4. Thermal stability of the enzyme solution was determined by assaying residual ac- tivity after heating the enzyme solution to various temperatures for fifteen minutes CO o o CO CO <I LU cr o LU O. CONTROL CMC 70 LOW CMC 70 MEDIUM CMC 70 HIGH 0 10 20 30 40 50 INCUBATION TIME (MINUTES) I-'K,I ]<]•. 1. Ki'tVrt of crystalline style enzyme on CMC 70 of different viso^ity. Final concentration of CMC /o was $ ; pi I was and tcinprratuiv S30 Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redfield, Alfred Clarence, 1890-1983. Woods Hole, Mass. : Marine Biological Laboratory


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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology