. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 70 J. E. DOELLER AND D. W. KRAUS a â n â¢3 o a. o J5 05. 500 520 540 560 580 600 Wavelength, nm Figure 1. The oxygen equilibrium spectrogram of Tellina ahernata cerebro-visceral connective neurohemoglobin in situ from (K oxyneu- rohemoglobin (deoxyneurohemoglobin) to 100% oxyneurohemo- globin. Intermediate spectra were taken at , , and mm Hg P02. The unusual deoxyneurohemoglobin spectrum exhibits two wavelength maxima. The spectrogram of Spisula solidissima neuro- hemoglobin in situ, collected at higher PO;, is
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 70 J. E. DOELLER AND D. W. KRAUS a â n â¢3 o a. o J5 05. 500 520 540 560 580 600 Wavelength, nm Figure 1. The oxygen equilibrium spectrogram of Tellina ahernata cerebro-visceral connective neurohemoglobin in situ from (K oxyneu- rohemoglobin (deoxyneurohemoglobin) to 100% oxyneurohemo- globin. Intermediate spectra were taken at , , and mm Hg P02. The unusual deoxyneurohemoglobin spectrum exhibits two wavelength maxima. The spectrogram of Spisula solidissima neuro- hemoglobin in situ, collected at higher PO;, is identical. chamber), which is well below the time required for neu- rohemoglobin deoxygenation. approximately 30 min. At 3-5 min intervals, transmitted light intensities at 600, 575, 560, and 540 nm were recorded. A deoxyneuro- hemoglobin spectrum was recorded when the light inten- sity trace reached an asymptote. Humidified air was then reintroduced to the sample chamber and the change in light intensity at 560 nm during reoxygenation of the neurohemoglobin was recorded. Neurohemoglobin frac- tional saturation, calculated as before, was plotted as a function of time. The oxygen unloading rate in situ was calculated as the product of the slope of this curve and the heme concentration. Oxygen dissociation using humidified CO as the deoxygenating gas was measured in a similar manner. Light intensities were collected at 570 and 535 nm, the peak wavelengths for carbon monoxide hemoglobin. In several experiments, a few crystals of dithionite were added to the seawater surrounding connective segments in a depression slide. The well was quickly sealed with a cover slip and absorption spectra were collected immedi- ately thereafter. Results Spectral characteristics, heme concentration, and molecular weight The oxyneurohemoglobin spectrum (see Fig. 1) and carbon monoxide neurohemoglobin spectrum (not shown) of T. alternate! and 5. solidissima cerebro-vis- ceral connectives are simila
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
