. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LOCALIZATION OF CHLOROPEROXIDASE 219. Figure 2. Immunofluorescence labeling of cross sections of the tail of Notomastus lobalus. Polyclonal antisera to heme protein and flavoprotein subunits of chloroperoxidase from N- hhalus were used; binding was visualized by fluorescein-conjugated anti-rabbit antibody. Labeling with (A) heme protein antibody; (B) flavoprotein antibody; (C) 1:1 mixture of heme protein and flavoprotein antibodies; (D) preimmune rabbit serum. (E) Neighboring section stained with hemotoxylin and eosin and ex
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. LOCALIZATION OF CHLOROPEROXIDASE 219. Figure 2. Immunofluorescence labeling of cross sections of the tail of Notomastus lobalus. Polyclonal antisera to heme protein and flavoprotein subunits of chloroperoxidase from N- hhalus were used; binding was visualized by fluorescein-conjugated anti-rabbit antibody. Labeling with (A) heme protein antibody; (B) flavoprotein antibody; (C) 1:1 mixture of heme protein and flavoprotein antibodies; (D) preimmune rabbit serum. (E) Neighboring section stained with hemotoxylin and eosin and examined with light microscopy (m—muscle, e—epidermis). (F) Same section as in (E), at higher magnification (m—muscle, e—epidermis). Scale bar in A = 72 jim and applies to A-E. Scale bar in F = 36 ^m. flavoprotein moiety was detected in the head region. In contrast to the immunocytochemical results, heme protein was detected in all three body regions (Table I). This could be due to nonspecific activity of the antiserum or to the actual presence of heme protein in all three body regions. If the latter, then the heme protein may have another function in the head and mid-body of the worm, but it must be complexed in some way such that we did not detect it by immunocytochemistry (Knapp el ai, 1991). Tests for nonspecific activity of the anti-heme protein serum were negative. When anti-heme protein serum preabsorbed with the purified heme protein moiety was reacted with crude extract and pure heme protein, no re- action was seen with either the purified heme protein. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine Biological Laboratory (Woods Hole, Mass. ). Annual report 1907/08-1952; Lillie, Frank Rattray, 1870-1947; Moore, Carl Richard, 1892-; Redf
Size: 1570px × 1592px
Photo credit: © Library Book Collection / Alamy / Afripics
License: Licensed
Model Released: No
Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
