Practical physiological chemistry : a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . C C CH V \/ CH NH Indoxyl. C,H,NO. CH CH //\ /\ HC C — C • 0 0 • C — C CH I I II I II + 2H20 HC C C C C CH V \/ \/ \// CH NH XH CH Indigo-blue. C18H10N502. 2. Obermayers Test.—Nearly fill a test-tube with a mix-ture composed of equal volumes of Obermayers reagent1 andthe urine under examination. Add 2-3 of chloroform,place the thumb over the end of the test-tube and shake thor-oughly. How does this compare with Jaffes test? C0NHCH2C00H. HIPPUR
Practical physiological chemistry : a book designed for use in courses in practical physiological chemistry in schools of medicine and of science . C C CH V \/ CH NH Indoxyl. C,H,NO. CH CH //\ /\ HC C — C • 0 0 • C — C CH I I II I II + 2H20 HC C C C C CH V \/ \/ \// CH NH XH CH Indigo-blue. C18H10N502. 2. Obermayers Test.—Nearly fill a test-tube with a mix-ture composed of equal volumes of Obermayers reagent1 andthe urine under examination. Add 2-3 of chloroform,place the thumb over the end of the test-tube and shake thor-oughly. How does this compare with Jaffes test? C0NHCH2C00H. HIPPURIC ACID, I \/ 1 Obermayers reagent is prepared by adding 2-4 grams of ferric chlo-ride to a liter of concentrated HC1 (sp. gr. ). 256 PHYSIOLOGICAL CHEMISTRY. This acid occurs normally in the urine of both the carnivoraand herbivora but is more abundant in the urine of the is formed by a synthesis of benzoic acid and glycocoll whichtakes place in the kidneys. The average excretion of an adultman for 24 hours under normal conditions is about acid crystallizes in needles or rhombic prisms (see Fig. Hippuric Acid. Fig. 92. above), the particular form depending upon the ra-pidity of crystallization. It is easily soluble in alcohol or hotwater, and only slightly soluble in ether. The output of hip-puric acid is increased in diabetes owing probably to the inges-tion of much proteid and fruit. It is decreased in fevers andin certain kidney disorders where the synthetic activity of therenal cells is diminished. Experiments on Hippuric Acid. 1. Separation from the Urine.—Render 500-1000 of urine of the horse or cow1 alkaline with milk of lime, boil for 1 If urine of the horse or cow is not available human urine may servethe purpose fully as well provided means are taken to increase its contentof hippuric acid. This may be conveniently accomplished by ingesting 2 URINE. 257 a few moments and filter while hot. Concentrate the filtrate,
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