. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 352 RALPH I. SMITH Kxposure In 5e/v Smith (1(H»7) lor Rhithropanopeus except that exposure' time was short- ened tn 15 iitinutes instead ol" 30, since C'arciiuis is considerably more permeable to I) than the inrmer crab. Seawater was diluted bv the addition of local Newcastle pnndwater plus sufficient I )L,( ) ((>() moles '/< } to yield 75, 50, 35 and 25% SW. Since the addition of the IXO to S\Y caused a dilution to 94% SW, crabs to be tested in this medium were placed in S\Y diluted to 94% SW with deionized wa


. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 352 RALPH I. SMITH Kxposure In 5e/v Smith (1(H»7) lor Rhithropanopeus except that exposure' time was short- ened tn 15 iitinutes instead ol" 30, since C'arciiuis is considerably more permeable to I) than the inrmer crab. Seawater was diluted bv the addition of local Newcastle pnndwater plus sufficient I )L,( ) ((>() moles '/< } to yield 75, 50, 35 and 25% SW. Since the addition of the IXO to S\Y caused a dilution to 94% SW, crabs to be tested in this medium were placed in S\Y diluted to 94% SW with deionized water 2-3 hours before the tests. Crabs above 15 g in weight were tested iu a liter of the 5r/r D2O solutions, smaller crabs in 500 ml. At the end of each exposure the crab was removed, quickly dried in a cloth towel, and a few drops of blood drawn by puncture of the arthrodial membrane at a leg base. This 100 </5 90- £ 80 — 50 c 'c 40 g | 30 "5 CO 02° Q i i i i i 345 10 Wet weight in grams 20 75% SW. 25-35%SW J L 30 40 50 FIGURE 1. Curves relating DuO-uptake (as % saturation attained in 15 min ) to wet weight of Cardans plotted by method of least squares. The equations—Uptake = a (weight)(6 —are: 94% SW, Uptake = /P"1 "r'L", n = 23; 75% SW, Uptake = W<"^0757), »=11; 50% SW, Uptake = //''-"I1L"", w = 12; 25-35% SW, Uptake = W^'ww\ n = 22. sample was immediately placed in the large end of a Pasteur pipette, the pipette closed with a small cork, and its tip sealed in a flame. These pipettes, containing samples of blood and medium, were laid on a slide warmer overnight at 50° C to distill the water from the samples into the tips, which projected several inches from the warmer and were cooled by the evaporation of water from a strip of facial tissue paper laid across them with its ends dipping into beakers of water. D^O contents of blood samples, controls, and the respective media were estimated by compa


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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology