. Culture methods for invertebrate animals;. Invertebrates -- Cultures and culture media; Invertebrates -- Collection and preservation. 416 Phylum Arthropoda sibility of drowning. It consists of a small beaker of water inverted over a semicircle of filter paper in a petri dish. [See p. 413.] As a food, cane sugar will support the flies indefinitely. In supply- ing sugar we have abandoned our earlier use of a molar solution in favor of lumps of domino sugar as suggested by Haub and Miller (loc. cit.). This is a vastly superior method. While cane sugar alone will support the flies, the produc- t
. Culture methods for invertebrate animals;. Invertebrates -- Cultures and culture media; Invertebrates -- Collection and preservation. 416 Phylum Arthropoda sibility of drowning. It consists of a small beaker of water inverted over a semicircle of filter paper in a petri dish. [See p. 413.] As a food, cane sugar will support the flies indefinitely. In supply- ing sugar we have abandoned our earlier use of a molar solution in favor of lumps of domino sugar as suggested by Haub and Miller (loc. cit.). This is a vastly superior method. While cane sugar alone will support the flies, the produc- tion of eggs requires protein. This we have supplied with fresh beef liver which also provides a favorable medium for oviposition. Every second day a small porcelain dish cm. loosely filled with liver, cut into approximately 2 cm. cubes and moistened \\/ \j with a few drops of water, is placed in the cage and left for 24 hours. The amount and subdivision of the liver insure moist surfaces in at least some crevices of the mass through- out the 24 hour interval, thus permitting feeding and oviposition.* Once the egg masses are laid, the eggs or newly hatched larvae are removed from the liver and placed in the gills of fish heads obtained from the fish market. The fish head is then placed in a wide-lipped porcelain soup plate and placed in the rearing can (Fig. 73)- The rearing can consists of a galvanized pail in the lid (1) of which a circular opening is cut. Into the opening a metal collar (co) is soldered containing a simple damper (d). Over the collar a removable cylindrical screen cage (ca) is fitted. The damper permits the regulation of moisture which is very important in the successful rearing of larvae. If conditions are too moist the larvae leave the food and migrate in all directions, a fact which must be familiar to all who have tried to rear these animals extensively. *The species above enumerated readily oviposit under the conditions described. An- other clo
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