. Laboratory work in bacteriology. Bacteriology. 176 BACTEEIOLOGY; ground plate of the ice apparatus, and cover with the bell- jar. As soon as the plate is cool, it is ready to receive the gelatin. Before pouring the contents of a tube upon a plate; it is necessary, as a matter of precaution, to sterilize the neck of the tube. This is easily accomplished, in the following manner: Remove the cotton plug from the tube (No. 1), and rapidly rotate the neck of the tube in the flame. This is thus sterilized, and the contents of the tube â can now be poured, without coming into contact with for- eign
. Laboratory work in bacteriology. Bacteriology. 176 BACTEEIOLOGY; ground plate of the ice apparatus, and cover with the bell- jar. As soon as the plate is cool, it is ready to receive the gelatin. Before pouring the contents of a tube upon a plate; it is necessary, as a matter of precaution, to sterilize the neck of the tube. This is easily accomplished, in the following manner: Remove the cotton plug from the tube (No. 1), and rapidly rotate the neck of the tube in the flame. This is thus sterilized, and the contents of the tube â can now be poured, without coming into contact with for- eign organisms. In about a minute, the neck of the tube is sufficiently cool to proceed. Raise the bell-jar some- what, shielding the plate as much as possible from draughts of air, and pour the gelatin on the center of the plate. With the lip of the tube, rapidly spread the gelatin over as much of the surface as possible, avoiding, however, the edges of the plate. The plate is now allowed to remain under the bell-jar till the gelatin be- comes solid. The empty gelatin tube should not be placed on the table but should be set in a tumbler. When through plating, all the empty tubes should be sterilized, either by immersion in mercuric chlo- ride, or better by exposure to boiling water or to steam. While the gelatin on the plate is becoming solid, a '' moist chamber " is prepared in the same way as for potato cultures (p. 167). It is not necessary to sterilize, either the moist chamber, or the glass benches on which the plates are to be placed. Instead of mercuric chloride, tap-water. Fig. 31. Ise apparatus for cooling gelatin Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Novy, Frederick George, 1864- . Ann Arbor [Mich. ] G. Wahr
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