. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CHLORIDE REGULATION AND RESPIRATION 261 to the laboratory, filtered and used in the experiments. It contained 480 milli- moles of chloride per liter, which conforms to the figure for normal sea water used by Cole (1940). The value is lower than that determined for some open sea samples. This fact may be accounted for considering that Rehoboth Beach, Dela- ware, is but a few miles south of the mouth of the Delaware River. Distilled water was used to obtain dilutions amounting to 360, 240, and 120 millimoles of chloride per li
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. CHLORIDE REGULATION AND RESPIRATION 261 to the laboratory, filtered and used in the experiments. It contained 480 milli- moles of chloride per liter, which conforms to the figure for normal sea water used by Cole (1940). The value is lower than that determined for some open sea samples. This fact may be accounted for considering that Rehoboth Beach, Dela- ware, is but a few miles south of the mouth of the Delaware River. Distilled water was used to obtain dilutions amounting to 360, 240, and 120 millimoles of chloride per liter. Evaporation in shallow pans under a fan at room temperature resulted in concentrations of 600 and 720 millimoles of chloride per liter. In addition to the sea water concentrations, some crabs were put into distilled water, which is recorded as 0 millimoles of chloride per liter. Others were not put into any of the experi- mental solutions but remained on damp sand and are recorded as "; The oxygen consumption of the crabs was measured in a respirometer of the direct reading volumetric type, similar in principle to the micro-respirometer of Scholander (1942). The apparatus is shown in Figure 1. The animal chamber. FIGURE 1. A volumetric respirometer (for explanation see text). (1) was constructed from a finger bowl, the rim of which was ground flat and smooth. An alkali cup (2) made by drilling out a piece of one inch plexiglass rod was fastened to the bottom of the bowl with DeKhotinsky cement. A shield of plastic screen (Lumenite) placed around the cup prevented the animal from getting into the alkali. The lid of the animal chamber (3) was a disc of quarter-inch plexiglass, recessed at the periphery to close on the ground rim of the finger bowl. Three holes were drilled in the disc and fitted with rubber stoppers as shown in the figure. A three-inch length of glass tubing through one of the peripheral stoppers served as a by-pass through which fluids were ad
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
