. Fungous diseases of plants : with chapters on physiology, culture methods and technique . Fungi in agriculture. 58 PHYSIOLOGICAL RELATIONS very careful work purified beeswax and white vaseline should be used. As a matter of convenience, two rings are usually cemented to the same slip. To make the cells, the wax is kept melted, the slide is slightly heated in the flame, and by means of the forceps the ring is passed through the flame, after which one edge is dipped lightly into the melted wax, then quickly placed upon the slip. If the wax is too hot, it will be nec- essary to touch the ring s
. Fungous diseases of plants : with chapters on physiology, culture methods and technique . Fungi in agriculture. 58 PHYSIOLOGICAL RELATIONS very careful work purified beeswax and white vaseline should be used. As a matter of convenience, two rings are usually cemented to the same slip. To make the cells, the wax is kept melted, the slide is slightly heated in the flame, and by means of the forceps the ring is passed through the flame, after which one edge is dipped lightly into the melted wax, then quickly placed upon the slip. If the wax is too hot, it will be nec- essary to touch the ring sev- eral times to the melted wax, then raise it high enough to cool somewhat. When the wax is cool the free edge of the cylinder is provided with a ring of vaseline by invert- ing the cell over a slip, or shallow watch crystal, upon which there is spread a thin layer of melted vaseline (Fig. Fig. 12. Stand and Dish for Beeswax , t-,, ,, , ,j i 12). The cell should be momentarily held in this inverted position, or rested in this position on a rack, in order that the ring of vaseline will have some depth. By means of the vaseline ring the cover is, at the proper time, cemented firmly to the glass cylinder. If the temperature at which the cultures are to be incubated differs considerably from that at which the cells are made, it has been found well to make with the back of a scalpel a small nick in the vaseline ring, through which nick the expanding air may pass when the cultures are placed in the thermostat, or culture incubator. Afterwards they may be permanently sealed by slight pressure with scalpel or needle. A drop of the culture fluid to be used is placed on each cover glass, and about half a dozen drops of the same fluid are placed in the bottom of the cell. A small glass rod is the only satisfactory dropper for the first-mentioned work. The drops are inoculated with a few spores by means of a platinum needle, massing or bunching of the spores being prevented as much as. P
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Keywords: ., bookauthorduggarbe, bookcentury1900, bookdecade1900, bookyear1909
