. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 206 S. Y. FENG establish a base line as the control for certain of the studies cited below. Ten oysters held at 1(> C. were bled nine times: 0, j. 7. 1. -\ 5, 10 and 20 hours after ihe start of the experiment. The grand total mean of leucocytes per ml. of blood based on (H) observations was X 10" with a standard error of and si \ - x) The statistical constants of leucoc\u> obtained at each time interval are summari/.ed in Table IV. Kach mean is com]tared with the grand total mean to ascertain whet
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 206 S. Y. FENG establish a base line as the control for certain of the studies cited below. Ten oysters held at 1(> C. were bled nine times: 0, j. 7. 1. -\ 5, 10 and 20 hours after ihe start of the experiment. The grand total mean of leucocytes per ml. of blood based on (H) observations was X 10" with a standard error of and si \ - x) The statistical constants of leucoc\u> obtained at each time interval are summari/.ed in Table IV. Kach mean is com]tared with the grand total mean to ascertain whether the difference between them is significant. The large /' value, in each case, suggests that the mean number of leucocytes at any time interval dur- ing the 20-hour period does not differ significantly from the population mean. Tin- data are also presented in graphic form ( Kig. 4). Concurrently with the above experiment, five oysters were each injected with ml. of sea water. Heart blood samples were obtained at the following intervals: ',. L :l. 2, 4, 8 and 96 hours after the injection. Significant changes in leucocyte numbers during the first H hours are observed (Fig. 4). Immediately after the injection, the number of circulating leucocyte's drops abruptly; it reaches the lowest point in .', hour and slowlv regains its normal number in about 1 \ hours. The sudden drop in leucocvte numbers after the injection of sea water is ascribed to the dis- placement of oyster blood in the ventricle by the inoculum or to dilution or to disturbance of heart rate or all three together. The gradual mixing of the inoculum with the influx of oyster blood containing many leucocytes results in the subsequent rise of leucocyte numbers. After 2 hours the number of leucocytes in this group • Repeated bleedings A Injected with sea water followed by repeated bleedings 99 % confidence limits. 0 HOURS I;K,I KK 4. Tin- effect of injection of sea \\ater and repeated bleedings on the number of l
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
