. Biological structure and function; proceedings. Biochemistry; Cytology. ENZYMK- FORMATION OF DEOXYRIBONUCLEIC ACID 105 that the labelled deoxyribonucleotide was subsequently used for DNA synthesis. In the experiment described in Fig. 2 DNA was synthesized. Fig. I. Time curve of formation from tritium or ''-P-labelled CMP, as measured by the incorporation of isotope into DNA [7, 8]. Incubation conditions for one time point: o • i ^tmole of labelled CMP, i o /^^mole of ATP, 5 -o /^imoles of MgCl,, o-i mg. of heated DNA (10 min. at 100 ) and 4-5 mg. of "enz\Tne", final volume 0-4
. Biological structure and function; proceedings. Biochemistry; Cytology. ENZYMK- FORMATION OF DEOXYRIBONUCLEIC ACID 105 that the labelled deoxyribonucleotide was subsequently used for DNA synthesis. In the experiment described in Fig. 2 DNA was synthesized. Fig. I. Time curve of formation from tritium or ''-P-labelled CMP, as measured by the incorporation of isotope into DNA [7, 8]. Incubation conditions for one time point: o • i ^tmole of labelled CMP, i o /^^mole of ATP, 5 -o /^imoles of MgCl,, o-i mg. of heated DNA (10 min. at 100 ) and 4-5 mg. of "enz\Tne", final volume 0-41 ml., pH 7 -4, 37^. CMP ?DNA t ^ t ' ^ Micrococcal DNA-se | Pancreatic DNA-se + spleen diesterase ,+venom diesterase \-® Md Total counts in CMP ' 500 dCMP 1900 7800 dAMP 3400 0 dCMP 2700 0 IMP 3900 0 Fig. 2. Enzymic degradation [9] of DNA formed from P*--CMP. 60 min. incubation with conditions as in Fig. i. from ^'-P-CMP and, after isolation, degraded enzymically in two different ways: (o) With pancreatic DNA-se + snake venom diesterase. These enzymes split DNA as indicated by the arrow in Fig. 2 and produce 5'-deoxy- ribonucleotides. Since 5'-labelled CMP was the precursor in the synthesis. Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original IUB/IUBS International Symposium (1st : 1960 : Stockholm); International Union of Biochemistry; International Union of Biological Sciences; Goodwin, T. W. (Trevor Walworth); Lindberg, Olov, 1914-. London, New York, Academic Press
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