. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 172 DOROTHY M. SKINNER A glance at Figure 4 shows that though the injection of ecdyson greatly in- creased the amount of labeled amino acid incorporated into protein, its effect was not much greater than the effect of injury inflicted by injection of Ringer's solu- tion (compare control 2 with the ecdyson animals). The specific activity of the blood proteins of the two pairs of animals which received injections prior to labeled valine (control and ecdyson animals) was only slightly higher than that of the pair which received
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. 172 DOROTHY M. SKINNER A glance at Figure 4 shows that though the injection of ecdyson greatly in- creased the amount of labeled amino acid incorporated into protein, its effect was not much greater than the effect of injury inflicted by injection of Ringer's solu- tion (compare control 2 with the ecdyson animals). The specific activity of the blood proteins of the two pairs of animals which received injections prior to labeled valine (control and ecdyson animals) was only slightly higher than that of the pair which received no prior "injury" (control 1) due to the first injection. On the other hand, two to nine times as much labeled amino acid was incorpo- rated into the other three tissues of the "injured" and "ecdyson" animals as into those of the uninjured animals. 6. Cytochrome c concentration in thoracic muscles The thoracic muscles begin developing 8 to 10 days after initiation of adult development (Schneiderman and Williams, 1953). However, in Figure 5 it can be seen that with the present methods of extraction and spectrophotometry at room temperature, cytochrome r was not detected until the seventeenth day of adult development. From the seventeenth day of development until the time of adult emergence, the concentration increased greatly. Therefore, the radioactivity of subcellular fractions of thoracic muscle was measured in animals on the seven- teenth and twenty-first days of adult development. 3000 C, -no injection C -injection of ringers E-injection of ecdyson. EPIDERMIS MID-GUT FAT BODY BLOOD FIGURE 4. Six brainless, diapausing pupae, two of which (Ci) were not injected; two of which (C») were injected with 10% methanol in Ringer's solution; and two of which (E) were injected with 4000 Calliphora units of ecdyson. Sixteen and one-half to 17 hours later, all 6 were injected with Dairies valine-1-C". All were sacrificed and their tissues removed
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
