. Elementary botany . Fig. 2. Fig- 3- Cell of spirogyra before treat- Cell of spirogyra after treatment +Uf* ctain TVip» merit with iodine. with alcohol and iodine. Hie Sldlll. 1 lie protoplasm remains uncolored. Now let us place these threads for a short time, two or three minutes, in strong alcohol, which kills the protoplasm. Then mount them in the eosin solution. The protoplasm now takes the eosin stain. After the proto- plasm has been killed we note that the nucleus is no longer elliptical or angular in outline, but is rounded. The strands of protoplasm are no longer in tension as they we
. Elementary botany . Fig. 2. Fig- 3- Cell of spirogyra before treat- Cell of spirogyra after treatment +Uf* ctain TVip» merit with iodine. with alcohol and iodine. Hie Sldlll. 1 lie protoplasm remains uncolored. Now let us place these threads for a short time, two or three minutes, in strong alcohol, which kills the protoplasm. Then mount them in the eosin solution. The protoplasm now takes the eosin stain. After the proto- plasm has been killed we note that the nucleus is no longer elliptical or angular in outline, but is rounded. The strands of protoplasm are no longer in tension as they were when alive. 10. Let us now take some fresh living threads and mount them in water. Place a small drop of dilute glycerine on the slip at one side of the cover glass, and with a bit of filter paper at the other side draw out the water. The glycerine will flow under the cover glass and come in contact with the spirogyra threads. Glycerine absorbs water promptly. Being in contact with the threads it draws water out of the cell cavity, thus caus-
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