. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. EXCYSTMENT IN BURSARIA TRUNCATELLA 89 In order to examine the nuclear relations within the cysts, some of the active bursarias from the culture dishes were allowed to encyst on cover slips. These were immersed in Schaudinn's fluid (with acetic) at different stages of encystment and the specimens were stained by the Feulgen method. Individuals in the early stages of encystment remained attached and stained well. Individuals with fully developed cyst membranes usually became detached during the acid hydrolysis and had to be ha
. The Biological bulletin. Biology; Zoology; Biology; Marine Biology. EXCYSTMENT IN BURSARIA TRUNCATELLA 89 In order to examine the nuclear relations within the cysts, some of the active bursarias from the culture dishes were allowed to encyst on cover slips. These were immersed in Schaudinn's fluid (with acetic) at different stages of encystment and the specimens were stained by the Feulgen method. Individuals in the early stages of encystment remained attached and stained well. Individuals with fully developed cyst membranes usually became detached during the acid hydrolysis and had to be handled from this point in embryological watch glasses. The impervious nature of the mature cyst membranes introduced difficulties in staining, so that the final preparations of mature cysts were barely adequate to reveal the nuclei. Later, when it became possible to activate the cysts dependably, some of them were fixed and stained at various stages of emergence. Whether a macronuclear reorganization occurs within the cysts was a point of special interest. If such a reorganization is present in ciliate cysts, it usually oc- curs in the early stages of encystment (Burt, Kidder and Claff, 1941; Beers, 1946). However, an examination of a considerable number of immature cysts (fixed 6-12 hours after isolation in spring water), as well as mature cysts (fixed 18, 24, or 48 hours after isolation), failed to reveal any evidence of a reorganization. Neverthe- less, brief mention of the complex configuration of the macronuclear loops and of the number and spatial distribution of the micronuclei seems appropriate, since these features of the cyst have received little more than incidental mention in the Please note that these images are extracted from scanned page images that may have been digitally enhanced for readability - coloration and appearance of these illustrations may not perfectly resemble the original Marine Biological Laboratory (Woods Hole, Mass. ); Marine
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Keywords: ., bookauthorlilliefrankrat, booksubjectbiology, booksubjectzoology
